T细胞专用U赢电竞毒

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原代T细胞的基因操作利器--U赢电竞毒

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T细胞及其特性简介

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? ? ?? T细胞(T?cell、T淋巴細胞/T?lymphocyte)是淋巴细胞的一种,在免疫反应中扮演着重要的角色。T细胞在胸腺内分化成熟,成熟后移居于周围淋巴组织。随着细胞免疫治疗的日益深入,T细胞的基因工程化改造显得越来越重要。

? ? ? ?然后,T细胞的基因操作却异常困难,普通的DNA质粒根本就转不进去,即使是病毒介导的基因转染操作,也有不少难题,主要体现在几个方面:

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1.常规的慢病毒、逆转录病毒,由于1)T细胞膜上受体的不稳定;2)非激活的T细胞本身无增殖能力;3)T细胞内部有阻碍反转录的机制等原因而导致慢病毒和逆转录病毒的T细胞感染能力偏弱,需要多次重复感染,且对于实验操作的条件要求十分苛刻。

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2.体外培养的T细胞本身十分敏感且脆弱,慢病毒感染对细胞的状态影响非常大。

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3.T细胞是悬浮细胞,进一步增大了病毒有效的难度。

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汉恒原代T细胞专用病毒---- CD4+ T细胞感染实例

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? ? ?至此汉恒拥有更强有力的T细胞感染工具--U赢电竞毒,堪称为原代T细胞的基因操作利器!若您有T细胞方面的实验问题,欢迎您来电咨询400-092-0065汉恒专业的技术团队会竭力为您答疑解惑。

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?参考文献

? ?1、Laterodorsal tegmentum interneuron subtypes oppositely regulate olfactory cue-induced innate fear;
? ? ?(2016年发表于Nature Neuroscience,作者:段树民)
? ?2、Endoplasmic reticulum stress induced?by tunicamycin and thapsigargin protects?against transient ischemic brain injury?Involvement of PARK2-dependent mitophagy;
? ? ?(2016年发表于Autophagy作者:Xiangnan Zhang)
? ?3、SNX13 reduction mediates heart failure through?degradative sorting of apoptosis repressor with?caspase recruitment domain;
? ? ?(2016年发表于Nature Communications,作者:陈义汉)
? ?4、TRB3 links insulin IGF to tumour promotion by interacting with p62 and impeding autophagic proteasomal degradations;
? ? ?(2016年发表于Nature Communications,作者:胡卓伟)
? ?5、Suppression of KRas-mutant cancer through the combined inhibition of KRAS with PLK1 and ROCK;
? ? ?(2016年发表于Nature Communications,作者:刘明耀)
? ?6、POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation;
? ? ?(2015年发表于Nature Communications,作者:Yongzhong Liu)
? ?7、LRP6 acts as a scaffold protein in cardiac gap?junction assembly;
? ? ?(2016年发表于Nature Communications,作者:陈义汉)
? ?8、Long-Range Chromosome Interactions Mediated by Cohesin Shape Circadian Gene Expression;
? ? ?(2016年发表于plos genetics,作者:Jun Yan )
? ?9、Histone deacetylase 4 selectively contributes to?podocyte injury in diabetic nephropathy;
? ? ?(2016年发表于Kidney International,作者:易凡)
? ?10、Roles of miR-1-1 and miR-181c in ventricular septal defects;
? ? ?(2016年发表于International Journal of Cardiology,作者:Li J)
? ?11、CCK-8S increased the filopodia and spines density in cultured hippocampal neurons of APP/PS1 and wild-type mice;
? ? ?(2013年发表于Neuroscience Letters,作者:王钦文)
? ?12、Interleukin-1β induces autophagy by affecting calcium homeostasis and trypsinogen activation in pancreatic acinar cells;
? ? ?(2013年发表于Int J Clin Exp Pathol ,作者:赵青川)
? ?13、MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31;
? ? ?(2014年发表于Oncology Reports,作者:Chaoyin Jiang)
? ?14、MicroRNA-216b/Beclin 1 axis regulates autophagy and apoptosis in human Tenon's capsule fibroblasts upon hydroxycamptothecin exposure;
? ? ?(2014年发表于Experimental Eye Research,作者: Yingbin Ge)
? ?15、Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathy;
? ? ?(2014年发表于Acta histochemica,作者: Qiuyue Wang)
? ?16、construction of recombinant adenovirus vector containing hBMP2 and hVEGF165 genes and its expression in rabbit Bone marrow mesenchymal stem cells;
? ? ?(2014年发表于Tissue and Cell,作者:Chun-yang Meng)
? ?17、Ulinastatin Reduces the Resistance of Liver Cancer Cells to Epirubicin by Inhibiting Autophagy;
? ? ?(2014年发表于plos one,作者:金钢、胡先贵)
? ?18、MicroRNA-204-5p–Mediated Regulation of SIRT1 Contributes to the Delay of Epithelial Cell Cycle Traversal in Diabetic Corneas;
? ? ?(2014年发表于iovs,作者:谢立信)
? ?19、Adenovirus siMDM2 and NDRG2 Gene Therapy Inhibits Cell Proliferation and Induces Apoptosis of Squamous Cell Carcinoma;
? ? ?(2015年发表于Cell Biochemistry and Biophysics,作者:Li Zhang)
? ?20、The Relevance of Nrf2 Pathway and Autophagy in Pancreatic Cancer Cells upon Stimulation of Reactive Oxygen Species;
? ? ?(2015年发表于Oxidative Medicine and Cellular Longevity,作者:Xin Shen)
? ?21、Overexpression of mitochondrial Hsp75 protects neural stem cells against microglia-derived soluble factor-induced neurotoxicity by regulating mitochondrial permeability transition pore opening in vitro;
? ? ?(2015年发表于International Journal of Molecular Medicine,作者:Yong Wang)
? ?22、SIRT6 suppresses phenylephrine-induced cardiomyocyte hypertrophy though inhibiting ;
? ? ?(2015年发表于Journal of Pharmacological Sciences,作者:刘培庆)
? ?23、Effects of insulin-like growth factor-1 on the assembly and secretion of very low-density lipoproteins in cow hepatocytes in vitro;
? ? ?(2015年发表于General and Comparative Endocrinology,作者:刘国文)
? ?24、SIRT6 suppresses isoproterenol-induced cardiac hypertrophy through activation of autophagy;
? ? ?(2015年发表于Translational Research,作者:刘培庆)
? ?25、Transcription factor E2-2 inhibits the proliferation of endothelial progenitor cells by suppressing autophagy;
? ? ?(2016年发表于International Journal of Molecular Medicine,作者:Hong Wang)
? ?26、Effect of adenovirus-mediated RNA interference of IL-1β expression on spinal cord injury in rats;
? ? ?(2016年发表于Spinal Cord)
? ?27、miR-23b-3p regulates the chemoresistance of gastric cancer cells by targeting ATG12 and HMGB2;
? ? ?(2016年发表于Cell Death and Disease,作者: Q Zhao)
? ?28、Adipose-derived stem cells induce autophagic activation and inhibit catabolic response to pro-inflammatory cytokines in rat chondrocytes;
? ? ?(2016年发表于Osteoarthritis and Cartilage,作者: Jian Zhang)
? ?29、β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy;
? ? ?(2016年发表于 Cell Death and Disease,作者:F Yi )
? ?30、MicroRNA-30a downregulation contributes to chemoresistance of osteosarcoma cells through activating Beclin-1-mediated autophagy;
? ? ?(2016年发表于Oncology Reports,作者:Lifeng Lao)
? ?31、Luteolin alleviates post‐infarction cardiac dysfunction by up‐regulating autophagy through Mst1 inhibition;
? ? ?(2016年发表于J. Cell. Mol. Med.,作者:Dongdong Sun )
? ?32、Long non-coding RNA HNF1A-AS1 functioned as an oncogene and autophagy promoter in hepatocellular carcinoma through sponging hsa-miR-30b-5p;
? ? ?(2016年发表于Biochemical and Biophysical Research Communications,作者: Jiahong Dong)
? ?33、Gambogic acid enhances the radiosensitivity of human esophageal cancer cells by inducing reactive oxygen species via targeting Akt/mTOR pathway;
? ? ?(2015年发表于Tumor Biology,作者:Xinchen Sun)
? ?34、Long non-coding RNA HNF1A-AS1 functioned as an oncogene and autophagy promoter in hepatocellular carcinoma through sponging hsa-miR-30b-5p;
? ? ?(发表于Biochemical and Biophysical Research Communications,作者:Jiahong Dong)
? ?35、miR-186 inhibits cell proliferation in multiple myeloma by repressing Jagged1;
? ? ?(2016年发表于Biochemical and Biophysical Research Communications,作者:Jun Peng)
? ?36、House dust mite extract induces growth factor expression in nasal mucosa by activating the PI3K/Akt/HIF-1α pathway;
? ? ?(2016年发表于Biochem Biophys Res Commun,作者:Zhou H)
? ?37、构建大鼠白细胞介素1β 基因shRNA U赢电竞毒载体;
? ? ?(2015年发表于中国组织工程研究 )
? ?38、Aldehyde dehydrogenase 2 activation in aged heart improves the autophagy by reducing the carbonyl modification on SIRT1;
? ? ?(2016年发表于Oncotarget,作者:Heng Ma)
? ?39、A novel role of microRNA 17-5p in the modulation of circadian rhythm;
? ? ?(2016年发表于Sci Rep,作者:Ji-Min Cao )
? ?40、Excess iodine promotes apoptosis of thyroid follicular epithelial cells by inducing autophagy suppression and is associated with Hashimoto thyroiditis disease;
? ? ?(2016年发表于J Autoimmun,作者:Xiao Y)
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